Creative Biolabs capture antibody and detection antibody
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Firstly, one known as the capture antibody is coated onto the surface of a 96-well plate to facilitate the immobilization of the target antigen. Then the detection antibody binds to the capture antibody-antigen complex. Finally, an enzyme-labeled secondary antibody conjugate specific for the detection antibody is added. In direct sandwich ELISA format, only two specific antibodies are used to sandwich the antigen. One is a capture antibody and detection antibody is another. Either monoclonal or affinity-purified polyclonal antibodies can be used as capture and detection antibodies, depending on cost, the dynamic range, and the sensitivity of the final assay.